Characterization, expression profiling, and functional analysis of a Populus trichocarpa defensin gene and its potential as an anti-Agrobacterium rooting medium additive.
Identifieur interne : 000A83 ( Main/Exploration ); précédent : 000A82; suivant : 000A84Characterization, expression profiling, and functional analysis of a Populus trichocarpa defensin gene and its potential as an anti-Agrobacterium rooting medium additive.
Auteurs : Hui Wei [République populaire de Chine] ; Ali Movahedi [République populaire de Chine] ; Chen Xu [République populaire de Chine] ; Weibo Sun [République populaire de Chine] ; Lingling Li [République populaire de Chine] ; Dawei Li [République populaire de Chine] ; Qiang Zhuge [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2019.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Agrobacterium (physiologie), Analyse de profil d'expression de gènes (MeSH), Antibactériens (pharmacologie), Antifongiques (pharmacologie), Céfotaxime (pharmacologie), Défensines (génétique), Défensines (métabolisme), Facteur de croissance végétal (pharmacologie), Gènes de plante (MeSH), Phénotype (MeSH), Populus (croissance et développement), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (microbiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Racines de plante (effets des médicaments et des substances chimiques), Racines de plante (microbiologie), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Spécificité d'organe (effets des médicaments et des substances chimiques), Spécificité d'organe (génétique), Stress physiologique (effets des médicaments et des substances chimiques), Stress physiologique (génétique), Tests de sensibilité microbienne (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Populus.
- effets des médicaments et des substances chimiques : Populus, Racines de plante, Régulation de l'expression des gènes végétaux, Spécificité d'organe, Stress physiologique.
- génétique : ARN messager, Défensines, Populus, Protéines végétales, Spécificité d'organe, Stress physiologique.
- microbiologie : Populus, Racines de plante.
- métabolisme : ARN messager, Défensines, Protéines végétales.
- pharmacologie : Antibactériens, Antifongiques, Céfotaxime, Facteur de croissance végétal.
- physiologie : Agrobacterium.
- Analyse de profil d'expression de gènes, Gènes de plante, Phénotype, Tests de sensibilité microbienne, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium (physiology), Anti-Bacterial Agents (pharmacology), Antifungal Agents (pharmacology), Cefotaxime (pharmacology), Defensins (genetics), Defensins (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (drug effects), Genes, Plant (MeSH), Microbial Sensitivity Tests (MeSH), Organ Specificity (drug effects), Organ Specificity (genetics), Phenotype (MeSH), Plant Growth Regulators (pharmacology), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (drug effects), Plant Roots (microbiology), Plants, Genetically Modified (MeSH), Populus (drug effects), Populus (genetics), Populus (growth & development), Populus (microbiology), RNA, Messenger (genetics), RNA, Messenger (metabolism), Stress, Physiological (drug effects), Stress, Physiological (genetics).
- MESH :
- chemical , genetics : Defensins, Plant Proteins, RNA, Messenger.
- chemical , metabolism : Defensins, Plant Proteins, RNA, Messenger.
- chemical , pharmacology : Anti-Bacterial Agents, Antifungal Agents, Cefotaxime, Plant Growth Regulators.
- drug effects : Gene Expression Regulation, Plant, Organ Specificity, Plant Roots, Populus, Stress, Physiological.
- genetics : Organ Specificity, Populus, Stress, Physiological.
- growth & development : Populus.
- microbiology : Plant Roots, Populus.
- physiology : Agrobacterium.
- Gene Expression Profiling, Genes, Plant, Microbial Sensitivity Tests, Phenotype, Plants, Genetically Modified.
Abstract
The diverse antimicrobial properties of defensins have attracted wide scientific interest in recent years. Also, antimicrobial peptides (AMPs), including cecropins, histatins, defensins, and cathelicidins, have recently become an antimicrobial research hotspot for their broad-spectrum antibacterial and antifungal activities. In addition, defensins play important roles in plant growth, development, and physiological metabolism, and demonstrate tissue specificity and regulation in response to pathogen attack or abiotic stress. In this study, we performed molecular cloning, characterization, expression profiling, and functional analysis of a defensin from Populus trichocarpa. The PtDef protein was highly expressed in the prokaryotic Escherichia coli system as a fusion protein (TrxA-PtDef). The purified protein exhibited strong antibacterial and antifungal functions. We then applied PtDef to rooting culture medium as an alternative exogenous additive to cefotaxime. PtDef expression levels increased significantly following both biotic and abiotic treatment. The degree of leaf damage observed in wild-type (WT) and transgenic poplars indicates that transgenic poplars that overexpress the PtDef gene gain enhanced disease resistance to Septotis populiperda. To further study the salicylic acid (SA) and jasmonic acid (JA) signal transduction pathways, SA- and JA-related and pathogenesis-related genes were analyzed using quantitative reverse-transcription polymerase chain reaction; there were significant differences in these pathways between transgenic and WT poplars. The defensin from Populus trichocarpa showed significant activity of anti-bacteria and anti-fungi. According to the results of qRT-PCR and physiological relevant indicators, the applied PtDef to rooting culture medium was chosen as an alternative exogenous additive to cefotaxime. Overexpressing the PtDef gene in poplar improve the disease resistance to Septotis populiperda.
DOI: 10.1038/s41598-019-51762-0
PubMed: 31653915
PubMed Central: PMC6814764
Affiliations:
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210037</wicri:regionArea><wicri:noRegion>210037</wicri:noRegion></affiliation></author><author><name sortKey="Movahedi, Ali" sort="Movahedi, Ali" uniqKey="Movahedi A" first="Ali" last="Movahedi">Ali Movahedi</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037</wicri:regionArea><wicri:noRegion>210037</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Chen" sort="Xu, Chen" uniqKey="Xu C" first="Chen" last="Xu">Chen Xu</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037</wicri:regionArea><wicri:noRegion>210037</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization, Nanjing Xiaozhuang University, Nanjing, 211171, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization, Nanjing Xiaozhuang University, Nanjing, 211171</wicri:regionArea><wicri:noRegion>211171</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Weibo" sort="Sun, Weibo" uniqKey="Sun W" first="Weibo" last="Sun">Weibo Sun</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037</wicri:regionArea><wicri:noRegion>210037</wicri:noRegion></affiliation></author><author><name sortKey="Li, Lingling" sort="Li, Lingling" uniqKey="Li L" first="Lingling" last="Li">Lingling Li</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037</wicri:regionArea><wicri:noRegion>210037</wicri:noRegion></affiliation></author><author><name sortKey="Li, Dawei" sort="Li, Dawei" uniqKey="Li D" first="Dawei" last="Li">Dawei Li</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037</wicri:regionArea><wicri:noRegion>210037</wicri:noRegion></affiliation></author><author><name sortKey="Zhuge, Qiang" sort="Zhuge, Qiang" uniqKey="Zhuge Q" first="Qiang" last="Zhuge">Qiang Zhuge</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China. qzhuge@njfu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037</wicri:regionArea><wicri:noRegion>210037</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium (physiology)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Antifungal Agents (pharmacology)</term><term>Cefotaxime (pharmacology)</term><term>Defensins (genetics)</term><term>Defensins (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (MeSH)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Organ Specificity (drug effects)</term><term>Organ Specificity (genetics)</term><term>Phenotype (MeSH)</term><term>Plant Growth Regulators (pharmacology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (drug effects)</term><term>Plant Roots (microbiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (microbiology)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Stress, Physiological (drug effects)</term><term>Stress, Physiological (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Agrobacterium (physiologie)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Antibactériens (pharmacologie)</term><term>Antifongiques (pharmacologie)</term><term>Céfotaxime (pharmacologie)</term><term>Défensines (génétique)</term><term>Défensines (métabolisme)</term><term>Facteur de croissance végétal (pharmacologie)</term><term>Gènes de plante (MeSH)</term><term>Phénotype (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Racines de plante (effets des médicaments et des substances chimiques)</term><term>Racines de plante (microbiologie)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term><term>Spécificité d'organe (effets des médicaments et des substances chimiques)</term><term>Spécificité d'organe (génétique)</term><term>Stress physiologique (effets des médicaments et des substances chimiques)</term><term>Stress physiologique (génétique)</term><term>Tests de sensibilité microbienne (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Defensins</term><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Defensins</term><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Antifungal Agents</term><term>Cefotaxime</term><term>Plant Growth Regulators</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Organ Specificity</term><term>Plant Roots</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Populus</term><term>Racines de plante</term><term>Régulation de l'expression des gènes végétaux</term><term>Spécificité d'organe</term><term>Stress physiologique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Organ Specificity</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Défensines</term><term>Populus</term><term>Protéines végétales</term><term>Spécificité d'organe</term><term>Stress physiologique</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Défensines</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term><term>Antifongiques</term><term>Céfotaxime</term><term>Facteur de croissance végétal</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Agrobacterium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agrobacterium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Genes, Plant</term><term>Microbial Sensitivity Tests</term><term>Phenotype</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Gènes de plante</term><term>Phénotype</term><term>Tests de sensibilité microbienne</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The diverse antimicrobial properties of defensins have attracted wide scientific interest in recent years. Also, antimicrobial peptides (AMPs), including cecropins, histatins, defensins, and cathelicidins, have recently become an antimicrobial research hotspot for their broad-spectrum antibacterial and antifungal activities. In addition, defensins play important roles in plant growth, development, and physiological metabolism, and demonstrate tissue specificity and regulation in response to pathogen attack or abiotic stress. In this study, we performed molecular cloning, characterization, expression profiling, and functional analysis of a defensin from Populus trichocarpa. The PtDef protein was highly expressed in the prokaryotic Escherichia coli system as a fusion protein (TrxA-PtDef). The purified protein exhibited strong antibacterial and antifungal functions. We then applied PtDef to rooting culture medium as an alternative exogenous additive to cefotaxime. PtDef expression levels increased significantly following both biotic and abiotic treatment. The degree of leaf damage observed in wild-type (WT) and transgenic poplars indicates that transgenic poplars that overexpress the PtDef gene gain enhanced disease resistance to Septotis populiperda. To further study the salicylic acid (SA) and jasmonic acid (JA) signal transduction pathways, SA- and JA-related and pathogenesis-related genes were analyzed using quantitative reverse-transcription polymerase chain reaction; there were significant differences in these pathways between transgenic and WT poplars. The defensin from Populus trichocarpa showed significant activity of anti-bacteria and anti-fungi. According to the results of qRT-PCR and physiological relevant indicators, the applied PtDef to rooting culture medium was chosen as an alternative exogenous additive to cefotaxime. Overexpressing the PtDef gene in poplar improve the disease resistance to Septotis populiperda.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31653915</PMID><DateCompleted><Year>2020</Year><Month>10</Month><Day>29</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>29</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>10</Month><Day>25</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>Characterization, expression profiling, and functional analysis of a Populus trichocarpa defensin gene and its potential as an anti-Agrobacterium rooting medium additive.</ArticleTitle><Pagination><MedlinePgn>15359</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-51762-0</ELocationID><Abstract><AbstractText>The diverse antimicrobial properties of defensins have attracted wide scientific interest in recent years. Also, antimicrobial peptides (AMPs), including cecropins, histatins, defensins, and cathelicidins, have recently become an antimicrobial research hotspot for their broad-spectrum antibacterial and antifungal activities. In addition, defensins play important roles in plant growth, development, and physiological metabolism, and demonstrate tissue specificity and regulation in response to pathogen attack or abiotic stress. In this study, we performed molecular cloning, characterization, expression profiling, and functional analysis of a defensin from Populus trichocarpa. The PtDef protein was highly expressed in the prokaryotic Escherichia coli system as a fusion protein (TrxA-PtDef). The purified protein exhibited strong antibacterial and antifungal functions. We then applied PtDef to rooting culture medium as an alternative exogenous additive to cefotaxime. PtDef expression levels increased significantly following both biotic and abiotic treatment. The degree of leaf damage observed in wild-type (WT) and transgenic poplars indicates that transgenic poplars that overexpress the PtDef gene gain enhanced disease resistance to Septotis populiperda. To further study the salicylic acid (SA) and jasmonic acid (JA) signal transduction pathways, SA- and JA-related and pathogenesis-related genes were analyzed using quantitative reverse-transcription polymerase chain reaction; there were significant differences in these pathways between transgenic and WT poplars. The defensin from Populus trichocarpa showed significant activity of anti-bacteria and anti-fungi. According to the results of qRT-PCR and physiological relevant indicators, the applied PtDef to rooting culture medium was chosen as an alternative exogenous additive to cefotaxime. Overexpressing the PtDef gene in poplar improve the disease resistance to Septotis populiperda.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Movahedi</LastName><ForeName>Ali</ForeName><Initials>A</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-5062-504X</Identifier><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Chen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization, Nanjing Xiaozhuang University, Nanjing, 211171, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Weibo</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Lingling</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Dawei</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhuge</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China. qzhuge@njfu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D023082">Defensins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010937">Plant Growth Regulators</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>N2GI8B1GK7</RegistryNumber><NameOfSubstance UI="D002439">Cefotaxime</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002439" MajorTopicYN="N">Cefotaxime</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D023082" MajorTopicYN="N">Defensins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="Y">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009928" MajorTopicYN="N">Organ Specificity</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010937" MajorTopicYN="N">Plant Growth Regulators</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>09</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31653915</ArticleId><ArticleId IdType="doi">10.1038/s41598-019-51762-0</ArticleId><ArticleId IdType="pii">10.1038/s41598-019-51762-0</ArticleId><ArticleId IdType="pmc">PMC6814764</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wei, Hui" sort="Wei, Hui" uniqKey="Wei H" first="Hui" last="Wei">Hui Wei</name></noRegion><name sortKey="Li, Dawei" sort="Li, Dawei" uniqKey="Li D" first="Dawei" last="Li">Dawei Li</name><name sortKey="Li, Lingling" sort="Li, Lingling" uniqKey="Li L" first="Lingling" last="Li">Lingling Li</name><name sortKey="Movahedi, Ali" sort="Movahedi, Ali" uniqKey="Movahedi A" first="Ali" last="Movahedi">Ali Movahedi</name><name sortKey="Sun, Weibo" sort="Sun, Weibo" uniqKey="Sun W" first="Weibo" last="Sun">Weibo Sun</name><name sortKey="Xu, Chen" sort="Xu, Chen" uniqKey="Xu C" first="Chen" last="Xu">Chen Xu</name><name sortKey="Xu, Chen" sort="Xu, Chen" uniqKey="Xu C" first="Chen" last="Xu">Chen Xu</name><name sortKey="Zhuge, Qiang" sort="Zhuge, Qiang" uniqKey="Zhuge Q" first="Qiang" last="Zhuge">Qiang Zhuge</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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